| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Café. |
|
Data corrente: |
08/03/2016 |
|
Data da última atualização: |
10/03/2016 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
YUYAMA, P. M.; REIS JUNIOR, O.; IVAMOTO, S. T.; DOMINGUES, D. S.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; CHARMETANT, P.; LEROY, T.; PEREIRA, L. F. P. |
|
Afiliação: |
PRISCILA MARY YUYAMA, UEL; OSVALDO REIS JÚNIOR, UNICAMP; SUZANA TIEMI IVAMOTO, UEL/IAPAR; DOUGLAS SILVA DOMINGUES, IAPAR/UNESP; MARCELO FALSARELLA CARAZZOLLE, UNICAMP; GONÇALO AMARANTE GUIMARÃES PEREIRA, UNICAMP; PIERRE CHARMETANT, CIRAD; THIERRY LEROY, CIRAD; LUIZ FILIPE PROTASIO PEREIRA, SAPC. |
|
Título: |
Transcriptome analysis in Coffea eugenioides, an Arabica coffee ancestor, reveals differentially expressed genes inleaves and fruits. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Molecular Genetics and Genomics, v. 291, n. 1, p. 323-336. 2016 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Studies in diploid parental species of polyploid plants are important to understand their contributions to the formation of plant and species evolution. Coffea eugenioides is a diploid species that is considered to be an ancestor of allopolyploid Coffea arabica together with Coffea canephora. Despite its importance in the evolutionary history of the main economic species of coffee, no study has focused on C. eugenioides molecular genetics. RNA-seq creates the possibility to generate reference transcriptomes and identify coding genes and potential candidates related to important agronomic traits. Therefore, the main objectives were to obtain a global overview of transcriptionally active genes in this species using next generation sequencing and to analyze specific genes that were highly expressed in leaves and fruits with potential exploratory characteristics for breeding and understanding the evolutionary biology of coffee. A de novo assembly generated 36,935 contigs that were annotated using eight databases. We observed a total of ~5000 differentially expressed genes between leaves and fruits. Several genes exclusively expressed in fruits did not exhibit similarities with sequences in any database. We selected ten differentially expressed unigenes in leaves and fruits to evaluate transcriptional profiles using qPCR. Our study provides the first gene catalog for C. eugenioides and enhances the knowledge concerning the mechanisms involved in the C. arabica homeologous. Furthermore, this work will open new avenues for studies into specific genes and pathways in this species, especially related to fruit, and our data have potential value in assisted breeding applications. MenosStudies in diploid parental species of polyploid plants are important to understand their contributions to the formation of plant and species evolution. Coffea eugenioides is a diploid species that is considered to be an ancestor of allopolyploid Coffea arabica together with Coffea canephora. Despite its importance in the evolutionary history of the main economic species of coffee, no study has focused on C. eugenioides molecular genetics. RNA-seq creates the possibility to generate reference transcriptomes and identify coding genes and potential candidates related to important agronomic traits. Therefore, the main objectives were to obtain a global overview of transcriptionally active genes in this species using next generation sequencing and to analyze specific genes that were highly expressed in leaves and fruits with potential exploratory characteristics for breeding and understanding the evolutionary biology of coffee. A de novo assembly generated 36,935 contigs that were annotated using eight databases. We observed a total of ~5000 differentially expressed genes between leaves and fruits. Several genes exclusively expressed in fruits did not exhibit similarities with sequences in any database. We selected ten differentially expressed unigenes in leaves and fruits to evaluate transcriptional profiles using qPCR. Our study provides the first gene catalog for C. eugenioides and enhances the knowledge concerning the mechanisms involved in the C. arabica homeologous. Furthe... Mostrar Tudo |
|
Palavras-Chave: |
Differentially expressed genes; Gene annotation; Homeologous; RNA-seq. |
|
Thesaurus Nal: |
Coffea. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140757/1/Transcriptome-analysis-in-coffea.pdf
|
|
Marc: |
LEADER 02549naa a2200277 a 4500
001 2040005
005 2016-03-10
008 2016 bl uuuu u00u1 u #d
100 1 $aYUYAMA, P. M.
245 $aTranscriptome analysis in Coffea eugenioides, an Arabica coffee ancestor, reveals differentially expressed genes inleaves and fruits.$h[electronic resource]
260 $c2016
520 $aStudies in diploid parental species of polyploid plants are important to understand their contributions to the formation of plant and species evolution. Coffea eugenioides is a diploid species that is considered to be an ancestor of allopolyploid Coffea arabica together with Coffea canephora. Despite its importance in the evolutionary history of the main economic species of coffee, no study has focused on C. eugenioides molecular genetics. RNA-seq creates the possibility to generate reference transcriptomes and identify coding genes and potential candidates related to important agronomic traits. Therefore, the main objectives were to obtain a global overview of transcriptionally active genes in this species using next generation sequencing and to analyze specific genes that were highly expressed in leaves and fruits with potential exploratory characteristics for breeding and understanding the evolutionary biology of coffee. A de novo assembly generated 36,935 contigs that were annotated using eight databases. We observed a total of ~5000 differentially expressed genes between leaves and fruits. Several genes exclusively expressed in fruits did not exhibit similarities with sequences in any database. We selected ten differentially expressed unigenes in leaves and fruits to evaluate transcriptional profiles using qPCR. Our study provides the first gene catalog for C. eugenioides and enhances the knowledge concerning the mechanisms involved in the C. arabica homeologous. Furthermore, this work will open new avenues for studies into specific genes and pathways in this species, especially related to fruit, and our data have potential value in assisted breeding applications.
650 $aCoffea
653 $aDifferentially expressed genes
653 $aGene annotation
653 $aHomeologous
653 $aRNA-seq
700 1 $aREIS JUNIOR, O.
700 1 $aIVAMOTO, S. T.
700 1 $aDOMINGUES, D. S.
700 1 $aCARAZZOLLE, M. F.
700 1 $aPEREIRA, G. A. G.
700 1 $aCHARMETANT, P.
700 1 $aLEROY, T.
700 1 $aPEREIRA, L. F. P.
773 $tMolecular Genetics and Genomics$gv. 291, n. 1, p. 323-336. 2016
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Café (CNPCa) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 32 | |
| 4. |  | BISWAS, A.; LIU, Z.; FURTADO, R. F.; ALVES, C. R.; CHENG, H. N. Novel polymeric products derived from biodiesel. In: CHENG, H. N.; MARYANOFF, C. A.; MILLER, B. D.; SCHMIDT, D. G. (Ed.). Stereochemistry and Global Connectivity: the Legacy of Ernest L. Eliel. Washington, DC: American Chemical Society, 2017. Cap. 12 v. 2 p. 207-220.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
|    |
| 5. | | BISWAS, A.; KIM, S.; BUTTRUM, M.; FURTADO, R. F.; ALVES, C. R.; CHENG, H. N. Preparation of hydrophobically modified cashew gum through reaction with alkyl ketene dimer. In: CHENG, H. N.; SMITH, P. B.; GROSS, R. A. (Ed.). Green polymer chemistry: new products, processes, and applications. Washington, DC: American Chemical Society, 2018. Cap. 10 p. 137-146 (ACS Symposium Series).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
|  |
| 6. | | GÓMEZ, A. V.; BISWAS, A.; TADINI, C. C.; FURTADO, R. F.; ALVES, C. R.; CHENG, H. N. Use of natural deep eutectic solvents for polymerization and polymer reactions. Journal of the Brazilian Chemical Society, Campinas, v. 30, n. 4, p. 717-726, Apr. 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| 7. | | CHENG, H. N.; FURTADO, R. F.; BISWAS, A.; ALVES, C.; PRIETO LOPEZ, C.; LAGARÓN CABELLO, J. M. Chemical modifications and applications of cashew byproducts: a selective review. ACS Food Science & Technology, v. 3, n. 4, p. 546–552, Apr. 2023.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| 9. | | OLIVEIRA, R. C. DE; ROMERO, F. B.; ALVES, C. R.; BISWAS, A.; CHENG, H. N.; FURTADO, R. F. Filmes de polianilina (pani) eletropolimerizados sobre superfície de ito para uso como indicador colorimétrico para detecção da amônia; polimerização de filmes de pani como indicador da presença de amõnia. Revista da Universidade Vale do Rio Verde, Três Corações, v. 17, n. 1, 9 p. jan./jul. 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| 10. | | BISWAS, A.; ALVES, C. R.; TREVISAN, M. T. S.; BERFIELD, J.; FURTADO, R. F.; LIU, Z.; CHENG, H. N. Derivatives of cardanol through the ene reaction with diethyl azodicarboxylate. Journal of the Brazilian Chemical Society, Campinas, v. 26, n. 7, p. 1078-1082, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| 11. | | CHENG, H. N.; FURTADO, R. F.; ALVES, C. R.; BASTOS, M. do S. R.; KIM, S.; BISWAS, A. Novel polyurethanes from xylan and TDI: Preparation and characterization. International Journal of Polymer Analysis and Characterization, v. 22, n. 1, p. 35-42, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| 12. | | BISWAS, A.; CHENG, H. N.; KIM, S.; APPELL, M.; BODDU, V. M.; ALVES, C. R.; FURTADO, R. F. Preparation of sorbitol-based polyurethanes and their semiinterpenetrating polymer networks. Journal of Applied Polymer Science, New York, v. 136, n. 22, artigo 47602, 8 p. 10 June 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| 13. | | BISWAS, A.; KIM, S.; FURTADO, R. F.; ALVES, C. R.; BETTRUM, M.; BODDU, V.; CHENG, H. N. Metal chloride-catalyzed acetylation of starch: Synthesis and characterization. International Journal of Polymer Analysis and Characterization, v. 23, n. 6, p. 577-589, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| 14. | | SILVA, L. C. DA; CASTELO, R. M.; CHENG, H. N.; BISWAS, A.; FURTADO, R. F.; ALVES, C. R. Methods of microencapsulation of vegetable oil: principles, stability and applications: a minireview. Food Technology & Biotechnology, v. 60, n. 3, Jul./Sept. 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| 15. | | ALEXANDRE, D. L.; MELO, A. M. A.; FURTADO, R. F.; BORGES, M. de F.; FIGUEIREDO, E. A. T.; BISWAS, A.; CHENG, H. N.; ALVES, C. R. Amperometric biosensor for Salmonella typhimurium detection in milk. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 25., 2016, Gramado. Anais... Gramado: sbCTA-RS, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| 16. | | BISWAS, A.; ALVES, C. R.; TREVISAN, M. T. S.; SILVA, R. L. E. da; FURTADO, R. F.; LIU, Z.; CHENG, H. N. Synthesis of a cardanol-amine derivative using an ionic liquid catalyst. Frontiers of Chemical Science and Engineering, v. 10, n. 3, p. 425-431, 2016.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| 17. | | OLIVEIRA, M. de A.; FURTADO, R. F.; BASTOS, M. do S. R.; BENEVIDES, S. D.; MUNIZ, C. R.; BISWAS, A.; CHENG, H. N. Cashew gum and gelatin blend for food packaging application. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 25., 2016, Gramado. Anais... Gramado: SBCTA-RS, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| 18. | | SILVA, L. C. DA; CASTELO, R. M.; MAGALHAES, H. C. R.; FURTADO, R. F.; CHENG, H. N.; BISWAS, A.; ALVES, C. R. Characterization and controlled release of pequi oil microcapsules for yogurt application. LWT. Food Science and Technology, v. 157, 113105, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| 19. | | ALEXANDRE, D. L.; MELO, A. M. A.; FURTADO, R. F.; BORGES, M. de F.; FIGUEIREDO, E. A. T.; BISWAS, A.; CHENG, H. N.; ALVES, C. R. A rapid and specific biosensor for Salmonella typhimurium detection in milk. Food and Bioprocess Technology, v. 11, n. 4, p. 748-756, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| 20. | | MELO, A. M. A.; ALEXANDRE, D. L.; FURTADO, R. F.; BORGES, M. de F.; FIGUEIREDO, E. A. T.; BISWAS, A.; CHENG, H. N.; ALVES, C. R. Electrochemical immunosensors for Salmonella detection in food. Applied Microbiology and Biotechnology, v. 100, n. 12, p. 5301-5312, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
| |
| Registros recuperados : 32 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
